Fluorescent oligonucleotides represent a special group and their synthesis and applications are covered in the section on the synthesis and properties of fluorescent oligonucleotides.
Biotin with different spacer arm lengths reduce steric hindrances associated olibonucleotide streptavidin binding and allow for efficient capturing of biotinylated proteins. By using this methodology we can incorporate amino-modifiers internally currently at pyrimidine nucleosides - C and U analogs within the oligonucleotide. Affinity chromatography and reconstitution into phospholipid vesicles. Nucleic Acid Library Construction. Figure 15 Schematic representation of indirect enzymic labelling.Biosynthesis offers several different Oligonucleotide biotinylation synthesis options. Avidin, streptavidin and other biotin-binding proteins have the ability to form. 13 Biotin-Labeled Oligonucleotides With Extraordinarily Long Tethering Arms A. Michael Morocho, Valeri Karamyshev, Olga Shcherbinina, and Nikolai Polushin. A series of oligonucleotides containing biotindUMP at various positions were synthesized and compared in quantitative, colorimetric.